Please use the following order form when submitting samples for Illumina HiSeq sequencing that require library construction.
If instead you are submitting Illumina compatible libraries, then please use the Illumina Sequencing form instead.
Illumina HiSeq Library Construction Services
GHTF offers several different library construction kits in order to create Illumina compatible libraries from a multitude of different sample types. For further inquiry on the library construction services provided, please use the following links to navigate to the sample type of interest: RNA-Sequencing, DNA-Sequencing.
Illumina HiSeq 4000 and HiSeq 2500 Library Submission Specifications
Users are able to submit self made libraries for sequencing as long as they are compatible with Illumina sequencers. However before libraries are sequenced, we recommend a series of quality check measures to ensure proper library quality and optimal clustering. Libraries should be quantified via Qubit Assay and/or qPCR. We recommend performing both methods of quantification to ensure proper cluster generation and to obtain the most reads. Libraries also should be run on the Bioanalyzer (or something equivalent) to get the average library size and to ensure that the library is as expected (free of adapter-dimers or other artifacts). Please provide libraries in 15ul volumes at 10nM concentrations.